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TAAs typically progress asymptomatically and can be life‐threatening when they rupture or dissect. Thoracic aortic aneurysm (TAA) refers to an irreversible enlargement of the aortic wall that exceeds 1.5 times the normal aortic diameter. Nonstandard Abbreviations and Acronyms α‐SMA Furthermore, RNA sequencing and immunostaining of the aortic valve revealed the upregulation of genes involved in endothelial‐to‐mesenchymal transition, inflammatory response, and tissue fibrosis in DKO valves and the presence of activated valve interstitial cells. DKO mice also showed progressive aortic valve thickening with collagen deposition from postnatal day 14, as well as turbulent flow in the ascending aorta. DKO mice showed the exacerbation of thoracic aortic aneurysms when compared with those of SMKO and upregulated Thbs1, a mechanical stress–responsive molecule, throughout the aorta. Knockdown of FBLN4 in human aortic ECs induced mesenchymal cell–like changes with the upregulation of mesenchymal genes, including TAGLN and MYL9. An evaluation of the thoracic aortic aneurysm phenotype and screening of altered signaling pathways were performed. Fbln4 double knockout ( DKO) mice in ECs and smooth muscle cells were generated and subjected to histological analysis, echocardiography, Western blotting, RNA sequencing, and immunostaining. Gene silencing of FBLN4 was conducted on human aortic ECs to evaluate morphological changes and gene expression profile. Stroke: Vascular and Interventional Neurology.Journal of the American Heart Association (JAHA).Circ: Cardiovascular Quality & Outcomes.Arteriosclerosis, Thrombosis, and Vascular Biology (ATVB). Media player codec pack download.com4/19/2023 However, according to the specs, Media Player Codec Pack is compatible with any media or audio player compatible with DirectShow. The default player is Windows Media Player Classic. 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